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AACC Approved Methods of Analysis, 11th Edition - AACC Method 39-01.01. Evaluation of NIR Instrument Calibration

AACC Approved Methods of Analysis, 11th Edition

Infrared Analysis

AACC Method 39-01.01
Evaluation of NIR Instrument Calibration


This procedure covers the general protocol to be adopted when an instrument’s calibration is statistically assessed. It assumes that reference and instrument values have been collected from a common set of samples by an appropriate method. By using regression analysis, the coefficients of the linear relationship between the instrument values and reference values of a set of standard samples are determined. When the reference values are regressed onto the instrument values, the slope is tested to determine whether it is significantly different from a 45° line (for skew), and if it is not significantly different, the significance of the bias (instrument mean minus reference mean) is tested. Where appropriate, precision and accuracy are also assessed according to the procedures specified in Method 39-00.01.

A spreadsheet calculator to evaluate NIR calibration accompanies this method.