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AACC International Approved Methods -AACC Method 86-90.01. B-Vitamins in Vitamin Concentrates by HPLC

AACC International Approved Methods

Vitamins

AACCI Method 86-90.01
B-Vitamins in Vitamin Concentrates by HPLC

VIEW METHOD

Objective
B-vitamins are dissolved in ionic-strength-adjusted aqueous acetic acid. They are separated by paired-ion high-performance liquid chromatography (HPLC) and quantitated by comparing their absorbance with absorbance of standard solutions at 285 nm. This method measures the presence and quantity of niacin, niacinamide, pyridoxine (vitamin B6), and riboflavin (vitamin B2) in vitamin concentrates containing a minimum of 0.1% of any one of the analytes.