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AACC Approved Methods of Analysis, 11th Edition -AACC Method 86-72.01. Riboflavin -- Microbiological Method

AACC Approved Methods of Analysis, 11th Edition

Vitamins

AACC Method 86-72.01
Riboflavin -- Microbiological Method

VIEW METHOD

Objective
The method measures riboflavin by a microbiological technique. Microorganisms such as Lactobacillus casei reproduce in proportion to the vitamin content in a culture medium. The resultant turbidity or metabolic products can be measured spectroscopically. In this method, the acidity resulting from microbial growth is measured by titration with a base (NaOH) and related to riboflavin content.