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AACC International Approved Methods -AACC Method 86-70.01. Riboflavin -- Fluorometric Method

AACC International Approved Methods

Vitamins

AACCI Method 86-70.01
Riboflavin -- Fluorometric Method

VIEW METHOD

Objective
Riboflavin, or vitamin B2, exhibits native fluorescence, a property used in its measurement. Ground sample is extracted in sulfuric acid; impurities are oxidized with permanganate; and the intensity of the extract's fluorescence is measured by a fluorometer. This method is applicable to whole-grain products, grits, meal, flaked and puffed cereals, farina, bread, etc.