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AACC International Approved Methods -AACC Method 86-45.01. Choline

AACC International Approved Methods

Vitamins

AACCI Method 86-45.01
Choline

VIEW METHOD

Objective
Choline (2-hydroxy-N,N,N-trimethylethanaminium hydroxide) is chemically measured as choline reineckate. Samples are extracted in methanol. Upon evaporation of methanol, choline is precipitated as reineckate, washed, dried, and reconstituted in acetone. The acetone solution of choline reineckate is spectroscopically measured at 526 nm.