METHODS
SHORTCUTS
ABOUT
FAQ
SUBSCRIBE
Methods Home
All Methods
Methods eXtras
Propose a Method
Cite a Method
Technical Committees
Archived Methods
Standards Affiliations
Suppliers Guide
Laboratory Proficiency Program
Harmonized Methods
AACC Approved Methods of Analysis, 11th Edition -AACC Method 86-45.01. Choline

AACC Approved Methods of Analysis, 11th Edition

Vitamins

AACC Method 86-45.01
Choline

VIEW METHOD

Objective
Choline (2-hydroxy-N,N,N-trimethylethanaminium hydroxide) is chemically measured as choline reineckate. Samples are extracted in methanol. Upon evaporation of methanol, choline is precipitated as reineckate, washed, dried, and reconstituted in acetone. The acetone solution of choline reineckate is spectroscopically measured at 526 nm.