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AACC International Approved Methods -AACC Method 86-05.01. Vitamin A and Carotene

AACC International Approved Methods

Vitamins

AACCI Method 86-05.01
Vitamin A and Carotene

VIEW METHOD

Objective
This method measures vitamin A and carotene in enriched cereals and mixed feeds. Ground samples are saponified with potassium hydroxide and extracted with hexane. Aliquots of hexane, containing the vitamin, are then applied to chromatographic columns. Carotene and vitamin A are eluted with 4% acetone (in hexane) and 15% acetone (in hexane), respectively, and read separately by a spectophotometer.