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AACC International Approved Methods -AACC Method 86-03.01. Vitamin A in Enriched Flour

AACC International Approved Methods

Vitamins

AACCI Method 86-03.01
Vitamin A in Enriched Flour

VIEW METHOD

Objective
This method measures vitamin A in enriched flour. Vitamin A reacts with chloroform saturated with antimony trichloride (Carr-Price reagent), resulting in a transient, blue-colored compound. Intensity of color is proportional to vitamin A content. The maximum intensity is at 620 nm. The method is not applicable for products containing carotene, as carotenoids also produce blue color with Carr-Price reagent. If carotenoids are present, they must be removed with the alumina column step. See Method 86-05.01.