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AACC Approved Methods of Analysis, 11th Edition -AACC Method 80-05.01. Determination of Saccharides by Liquid Chromatography

AACC Approved Methods of Analysis, 11th Edition

Sugars

AACC Method 80-05.01
Determination of Saccharides by Liquid Chromatography

VIEW METHOD

Objective
Corn syrup solution is passed through a metal ion-modified cation exchange column. Individual sugars are separated by molecular exclusion and ligand exchange. Eluted sugars are detected using a differential refractometer, and resulting peaks are quantitated against an appropriate standard with the aid of an electronic integrator. This method is applicable to all corn syrups, including those containing fructose, corn sugars, and starch hydrolysates (prepared by acid and/or enzyme conversion). See Note 1. This method has been adapted from the Standard Analytical Methods of the Corn Refiners Asociation, Inc. (CRA). See Ref.