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AACC International Approved Methods - AACC Method 46-14.03. Crude Protein -- Udy Dye Method

AACC International Approved Methods

Nitrogen

AACCI Method 46-14.03
Crude Protein -- Udy Dye Method

VIEW METHOD

Objective
This method measures protein content of wheat flour or ground wheat by colorimetric determination of unbound Acid Orange 12 dye. Ground sample is shaken with dye solution to form insoluble dye complex. After reaction with protein, dye concentrations are inversely correlated with percent protein in sample. This method is applicable to cereal grains, oilseeds, legumes, forages, animal products, dairy products, and formulated feeds or foods. It is not applicable to random mixtures. See Note 1.