METHODS
SHORTCUTS
ABOUT
FAQ
SUBSCRIBE
Methods Home
All Methods
Methods eXtras
Propose a Method
Cite a Method
Technical Committees
Archived Methods
Standards Affiliations
Suppliers Guide
Check Sample Program
Harmonized Methods
AACC International Approved Methods - AACC Method 45-25.01. Identification of Aflatoxin on TLC Plates

AACC International Approved Methods

Mycotoxins

AACCI Method 45-25.01
Identification of Aflatoxin on TLC Plates

VIEW METHOD

Objective
This method identifies aflatoxins B1 and G1 in partially purified extracts of grains, nuts, oilseeds, and other agricultural commodities by formation of water-adduct derivatives (aflatoxins B2a and G2a) on thin-layer chromatography (TLC) plates. Although their effects on humans are not known at present, aflatoxins are extremely potent carcinogens to a number of animals and should be regarded as potentially hazardous to humans. Whenever possible, perform operations under a hood and use a glove box when toxins are in a dry state because of their electrostatic nature. Use gloves and a mask for personal protection when handling toxin or highly contaminated commodities. Swab accidental spills with 5% sodium hypochlorite bleach and rinse glassware with bleach before washing to decontaminate.