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AACC International Approved Methods - AACC Method 45-16.01. Aflatoxin -- Liquid Chromatography Method

AACC International Approved Methods

Mycotoxins

AACCI Method 45-16.01
Aflatoxin -- Liquid Chromatography Method

VIEW METHOD

Objective
Aflatoxins are extracted and purified, derivatized with trifluoroacetic acid (TFA) (aflatoxins B1 and G1 to B2a and G2a, respectively), separated by reversed-phase high-performance liquid chromatography (HPLC), and detected by fluorescence. The method can measure 0.1 ng of aflatoxins B1, B2, G1, and G2. The detection limit is about 0.3 ng/g for each aflatoxin. This method is applicable to determination of aflatoxins B1, B2, G1, and G2 at >=13 ng total aflatoxin per g or 5 ng aflatoxin B1 per g in corn and peanut butter.