METHODS
SHORTCUTS
ABOUT
FAQ
SUBSCRIBE
Methods Home
All Methods
Methods eXtras
Propose a Method
Cite a Method
Technical Committees
Archived Methods
Standards Affiliations
Suppliers Guide
Check Sample Program
Harmonized Methods
AACC International Approved Methods - AACC Method 45-01.01. Sampling Grain for Mycotoxins

AACC International Approved Methods

Mycotoxins

AACCI Method 45-01.01
Sampling Grain for Mycotoxins

VIEW METHOD

Objective
This method provides a realistic approach for sampling corn, small grains, oilseeds, and pulses for mycotoxins. Mycotoxin contamination of cereal grains is likely to occur in pockets of high concentration, randomly distributed. Levels as high as 100,000 ppb have been reported in a single corn kernel. The U.S. Food and Drug Administration (FDA) has determined that the action level of mycotoxin is 20 ppb. One infected kernel of corn (with 100,000 ppb aflatoxin) may bring a sample of 5000 kernels (about 1500 g) to the FDA action level. Therefore, to try to make each 1.5-g sample representative, a contaminated seed should be reduced to about 1000 pieces. For this reason, the sampling and sample preparation guidelines must be carefully followed.