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AACC International Approved Methods - AACC Method 39-10.01. Near-Infrared Reflectance Method for Protein Determination in Small Grains

AACC International Approved Methods

Infrared Analysis

AACCI Method 39-10.01
Near-Infrared Reflectance Method for Protein Determination in Small Grains

VIEW METHOD

Objective
Protein content in small grains may be ascertained by near-infrared reflectance readings of the ground material at either discrete nonadjoining wavelengths or in a continuous wavelength region. Measurement is based upon a calibration to a suitable standard method (Method 46-11.02, 46-12.01, 46-13.01, 46-16.01, 46-19.01, or 46-30.01), whereupon an empirical model is made that relates spectral response to reference value. Near-infrared absorption, attributable to combination or overtone vibrational frequencies of NH from peptide linkages between amino acids, is the primary basis of a protein content model.