AACC International Approved Methods - AACC Method 32-23.01. beta-Glucan Content of Barley and Oats -- Rapid Enzymatic Procedure
AACC International Approved Methods
AACCI Method 32-23.01
beta-Glucan Content of Barley and Oats -- Rapid Enzymatic Procedure
(1-3)(1-4)-beta-D-glucan is determined using lichenase to generate soluble mixed-linkage beta-oligosaccharides, which are separated from insoluble polysaccharides by centrifugation. The oligosaccharides are hydrolyzed to glucose with beta-glucosidase. Subsequent colorimetric measurement of the derived glucose is performed using glucose oxidase/peroxidase reagent. The method is applicable to flours from whole oat, barley, and rye grains, milling fractions, and unsweetened cereal products. With minor modification (Ref. 1), the method may be used for cereal products containing high levels of simple sugars (glucose, sucrose, fructose, and corn sweeteners). About 20-30 samples may be analyzed in 2 hr.
A video demonstration accompanies this method.