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AACC International Approved Methods - AACC Method 32-22.01. β-Glucan in Oat Fractions and Unsweetened Oat Cereals

AACC International Approved Methods

Fiber

AACCI Method 32-22.01
β-Glucan in Oat Fractions and Unsweetened Oat Cereals

VIEW METHOD

Objective
This is a method to determine β-glucan content in oat fractions and unsweetened oat cereals via an enzymatic procedure using lichenase and β-glucosidase enzymes for two stage hydrolysis. The (1→3)(1→4)-β-D-glucan is converted to glucose, which is subsequently measured using the glucose oxidase/peroxidase procedure with visible spectrophotometry.