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AACC International Approved Methods - AACC Method 32-21.01. Insoluble and Soluble Dietary Fiber in Oat Products -- Enzymatic-Gravimetric Method

AACC International Approved Methods

Fiber

AACCI Method 32-21.01
Insoluble and Soluble Dietary Fiber in Oat Products -- Enzymatic-Gravimetric Method

VIEW METHOD

Objective
This method describes the determination of insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) fractions in oats and oat products. The procedures are an extension of AACC Method 32-05 and AOAC International Method 985.29 for total dietary fiber determination. Duplicate samples are gelatinized and then enzymatically digested with protease and amyloglucosidase to remove protein and starch.

Insoluble dietary fiber
Residue is filtered and washed with distilled water. Filtrate and wash are saved. Residue (IDF) is washed with 95% ethanol and acetone. After drying, residue is weighed. One of duplicates is analyzed for protein; the other is incinerated at 525° and ash is determined. IDF is weight of residue minus weight of protein and ash.

Soluble dietary fiber
Four volumes of 95% ethanol are added to combined filtrate and water washing to precipitate SDF. Precipitate is filtered and washed with 78% EtOH, 95% EtOH, and acetone. After drying, residue is weighed. One of duplicates is analyzed for protein; the other is incinerated at 525° and ash is determined. SDF is weight of residue minus weight of protein and ash.