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AACC International Approved Methods - AACC Method 32-07.01. Soluble, Insoluble, and Total Dietary Fiber in Foods and Food ProductsAACC International Approved Methods - AACC Method 32-07.01. Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products

AACC International Approved Methods

Fiber

AACCI Method 32-07.01
Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products

VIEW METHOD

Objective
This method is a modification of Methods 32-05 and 32-21. The MES-TRIS buffer substitutes for phosphate buffer in this method. See Note 1. Duplicate 1-g dried food samples are briefly subjected to sequential enzymatic digestion by heat-stable alpha-amylase, protease, and amyloglucosidase. This method determines soluble, insoluble, and total dietary fiber (TDF) content in processed foods and raw materials, such as cereal products, fruits, and vegetables.

Insoluble dietary fiber (IDF) is filtered, and the residue is washed with warm distilled water. A solution combining filtrate and water washings is precipitated with 4 volumes 95% EtOH for soluble dietary fiber (SDF) determination. The precipitate is then filtered and dried. Both SDF and IDF residues are corrected for protein, ash, and blank for final calculation of SDF and IDF values.

SDF is precipitated with EtOH, and the residue is then filtered, dried, and weighed. TDF value is corrected for protein and ash.