METHODS
SHORTCUTS
ABOUT
FAQ
SUBSCRIBE
Methods Home
All Methods
Methods eXtras
Propose a Method
Cite a Method
Technical Committees
Archived Methods
Standards Affiliations
Suppliers Guide
Check Sample Program
Harmonized Methods
AACC International Approved Methods - AACC Method 32-05.01. Total Dietary Fiber

AACC International Approved Methods

Fiber

AACCI Method 32-05.01
Total Dietary Fiber

VIEW METHOD

Objective
Total dietary fiber (TDF) is determined by gelatinizing duplicate samples (previously fat-extracted if fat content is >10%) with heat-stable alpha-amylase, digesting with protease and amyloglucosidase to remove protein and starch, and diluting the aqueous digest with four volumes ethanol to precipitate soluble dietary fiber. The residue is filtered; washed with 78% ethanol, 95% ethanol, and acetone; dried; and weighed. One duplicate is analyzed for protein, the other incinerated at 525° to determine ash. The TDF collected is corrected for method blanks, which include protein and ash determinations. This method is applicable to cereal grains and cereal-based products for nutritional labeling purposes.