Methods Home
All Methods
Methods eXtras
Propose a Method
Cite a Method
Technical Committees
Archived Methods
Standards Affiliations
Suppliers Guide
Laboratory Proficiency Program
Harmonized Methods
AACC Approved Methods of Analysis, 11th Edition - AACC Method 28-85.01. Ultraviolet Light Examination, for Rodent Urine

AACC Approved Methods of Analysis, 11th Edition

Extraneous Matter

AACC Method 28-85.01
Ultraviolet Light Examination, for Rodent Urine


Presence of rodent urine on bags and other packaging materials can be detected by examination for fluorescence under ultraviolet (UV) light. Stained spots and areas usually fluoresce with blue-white color, but color may vary, depending on background color of packaging material, type of lamp, and type of filter used. Other materials give similar fluorescence. Lubricating oils often leave fluorescence along seams or elsewhere on cloth bags. Fluorine materials and floor waxes also fluoresce and may impart fluorescence when bags are dragged across these materials or when they are spilled on bags. Urine fluoresces on grain seeds, but it is difficult to separate this fluorescence from natural fluorescence of certain seed tissues. Thus, the presence of UV fluorescence is a valuable means of locating areas that may be urine-stained, but positive identification can be made only by performing specific identification tests.