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AACC International Approved Methods - AACC Method 22-62.01. Measurement of Proteolytic Activity—Spectrophotometric Method

AACC International Approved Methods

Enzymes

AACCI Method 22-62.01
Measurement of Proteolytic Activity—Spectrophotometric Method

VIEW METHOD

Objective
This method estimates proteolytic enzyme activity in active proteinase preparations. The method is based on enzymatic hydrolysis of denatured hemoglobin at pH 4.7, followed by precipitation of unhydrolyzed substrate with trichloroacetic acid. Solubilized hemoglobin is then measured spectrophotometrically. Ratio of enzyme concentration to absorbance is linear within range of assay.