AACC Approved Methods of Analysis, 11th Edition - AACC Method 22-62.01. Measurement of Proteolytic Activity—Spectrophotometric
Method
AACC Approved Methods of Analysis, 11th Edition
Enzymes
AACC Method 22-62.01
Measurement of Proteolytic Activity—Spectrophotometric
Method
VIEW METHOD
Objective
This method estimates proteolytic enzyme activity in
active proteinase preparations. The method is based on enzymatic hydrolysis of
denatured hemoglobin at pH 4.7, followed by precipitation of unhydrolyzed
substrate with trichloroacetic acid. Solubilized hemoglobin is then measured
spectrophotometrically. Ratio of enzyme concentration to absorbance is linear
within range of assay.
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